Application of a 16S rRNA PCR-high-resolution melt analysis assay for rapid detection of Salmonella Bacteremia.

نویسندگان

  • Kevin Jeng
  • Samuel Yang
  • Helen Won
  • Charlotte A Gaydos
  • Yu-Hsiang Hsieh
  • Alex Kecojevic
  • Karen C Carroll
  • Justin Hardick
  • Richard E Rothman
چکیده

Current culture and phenotypic protocols for diagnosing Salmonella infections can be time-consuming. Here, we describe the application of a 16S rRNA PCR coupled to high-resolution melt analysis (HRMA) for species and serotype identification within 6 h of blood sample collection from a patient with Salmonella enterica serotype Enteritidis bacteremia.

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عنوان ژورنال:
  • Journal of clinical microbiology

دوره 50 3  شماره 

صفحات  -

تاریخ انتشار 2012